The first report of a protease resistant form of PrP in the urine of animals and humans affected with TSE is quite notable.

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Do you have a septic system? PrPRes in TSE Urine

The first report of a protease resistant form of PrP in the urine of animals and

humans affected with TSE is quite notable.

 

PrPRes was found in the urine of:

 

hamsters inoculated with Scrapie

as early as 17d post inoculation (long before clinical and pathological

changes)

(however, not between 35 and 49d post inoculation)

these findings may indicate clearance of inoculated PrPSc at day 17

 

in increasing amount from 56d post inoculation until clinical signs

appeared (day 105)

 

cattle with BSE

humans with CJD

E200K mutation

 

 

PrPRes was not found in the urine of:

 

normal hamsters

normal cattle

normal humans

 

The protein found in urine was entitled UPrPSc by the authors.

 

UPrPSc appears to differ from brain-derived PrPSc.

 

When UPrPSc 27-30 (from sedemented, dialysed and proteinase K digested urine)

was inoculated

hamsters did not develop clinical symptoms even after 270 d

hamsters did have UPrPSc in their urine

thus the UPrPSc 27-30 was transmitted and replicated within the new

hosts

 

 

When brain-derived PrPSc (diluted non-digested brain) was inoculated

hamsters elicited clinical symptoms after 80d

hamsters had UPrPSc in their urine

thus the brain-derived PrPSc was transmitted, replicated and pathogenic

within the new hosts

 

 

 

Thus UPrPSc 27-30 may:

 

result in subclinical tranmission; i.e. animals that are 'carriers' only

whether, these 'carriers' can tranmit the disease has yet to be

determined.

 

have a considerably delayed onset of clinical signs versus brain-derived

PrPSc

whether this is due to differences in the intrinsic transmissibility of

urinary or brain-derived PrPSc or from the digestion of urinary PrPSc and not

the brain-derived protein has yet to be determined

extended digestion of, aggregated, brain-derived PrPSc with proteinase

K decreases transmissibility , the sensitivity of urine-derived (probably

non-aggregated) PrPSc to proteinase K is not known

 

 

 

Together the findings above may allow us to:

 

 

develop an assay to screen for animals or humans exposed to PrPSc (test for

urinary clearance of consumed PrPSc)

for instance, this may be important if food is discovered that is

contaminated with PrPBSE

humans could be screened to confirm or alleviate fears of exposure to

prion disease

 

 

develop an early marker of TSE transmission in animals

begin treatment of humans, either exposed to PrPSc or with genetic defects,

before clinical signs are evident

once a treatment is available

 

identify animals that may 'carry' a prion disease but may never express

symptoms

 

 

 

 

karl theis jr

 

 

http://groups.msn.com/exposureofthetruth

 

madcowcoverup-

 

theoneswithoutnames-

 

 

 

 

 

 

 

 

 

 

 

 

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