Antioxidant activity of Smoke Shield in-vitro and in-vivo.

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Antioxidant activity of Smoke Shield in-vitro and in-vivo.

JoAnn Guest

Oct 20, 2005 14:06 PDT

 

Antioxidant activity of Smoke Shield in-vitro and in-vivo.

Sreekanth KS, Sabu MC, Varghese L, Manesh C, Kuttan G, Kuttan R.

 

Amala Cancer Research Centre, Amala Nagar, Thrissur 680 553, Kerala,

India.

 

Smoke Shield is a proprietory formulation containing extract of

turmeric

(Curcuma longa), obtained by supercritical carbon dioxide gas

extraction

and post-supercritical hydroethanolic extraction, together with

extracts

of green tea and other spices whose presence synergistically

increases

the activity of turmeric. This study evaluates the antioxidant

potentials of Smoke Shield in-vitro and in experimental animals, as

well

as in human models. Smoke Shield was found to scavenge superoxide

radicals generated by photoreduction of riboflavin (50% inhibitory

concentration = 91 microg mL(-1)) and hydroxyl radicals generated by

Fenton reaction (50% inhibitory concentration = 95 microg mL(-1))

and

reduced lipid peroxidation. Administration of Smoke Shield to mice

was

found to elevate antioxidant enzymes such as catalase and superoxide

dismutase in blood as well as in liver and kidney.

Glutathione-S-transferase activity was found to be significantly

elevated in liver and kidney of animals treated with Smoke Shield.

Glutathione levels were also significantly elevated in blood.

Glutathione reductase was significantly elevated in kidney.

Administration of Smoke Shield decreased the lipid peroxidation in

serum, liver and kidney, as well as reduced the levels of conjugated

dienes and hydroperoxides. Administration of Smoke Shield to smokers

was

found to increase the superoxide dismutase and glutathione in blood

and

decrease glutathione peroxidase. Smoke Shield inhibited phase I

enzymes

as represented by aniline-hydroxylase and aminopyrenedemethylase

in-vitro. These results indicate that Smoke Shield has potent

antioxidant activity, could inhibit phase I enzymes and increase

detoxifying enzymes, which makes it an effective chemoprotective

herbal

formulation.

 

PMID: 12841947 [PubMed - indexed for MEDLINE]

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?

cmd=Retrieve & db=pubmed & dopt=Abstract & list_uids=12841947 & query_hl=1

 

 

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