Borrelia burgdorferi Stimulates Macrophages to Secrete Higher Levels of Cytokine

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_J Infect Dis._

(javascript:AL_get(this,%20'jour',%20'J%20Infect%20Dis.') 2009 Dec

15;200(12):1936-1943.

Borrelia burgdorferi Stimulates Macrophages to Secrete Higher Levels of

Cytokines and Chemokines than Borrelia afzelii or Borrelia garinii.

_Strle K_

(http://www.ncbi.nlm.nih.gov/pubmed?term= " Strle%20K " [Author] & itool=EntrezSystem2\

..PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstract) ,

_Drouin EE_

(http://www.ncbi.nlm.nih.gov/pubmed?term= " Drouin%20EE " [Author] & itool=EntrezSyste\

m2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstract) ,

_Shen S_

(http://www.ncbi.nlm.nih.gov/pubmed?term= " Shen%20S " [Author] & itool=EntrezSystem2.\

PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstract) , _Khoury

JE_

(http://www.ncbi.nlm.nih.gov/pubmed?term= " Khoury%20JE " [Author] & itool=EntrezSyste\

m2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstract) , _McHugh

G_

(http://www.ncbi.nlm.nih.gov/pubmed?term= " McHugh%20G " [Author] & itool=EntrezSystem\

2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstract) ,

_Ruzic-Sabljic E_

(http://www.ncbi.nlm.nih.gov/pubmed?term= " Ruzic-Sabljic%20E " [Author] & itool=Entre\

zSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstract) ,

_Strle F_

(http://www.ncbi.nlm.nih.gov/pubmed?term= " Strle%20F " [Author] & itool=EntrezSystem2\

..PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstract) ,

_Steere AC_

(http://www.ncbi.nlm.nih.gov/pubmed?term= " Steere%20AC " [Author] & itool=EntrezSyste\

m2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstract) .

Center for Immunology and Inflammatory Diseases, Division of Rheumatology,

Allergy and Immunology, 2Infectious Diseases Division, Massachusetts

General Hospital, Harvard Medical School, Boston, Massachusetts; and 3Institute

of Microbiology and Immunology, University of Ljubljana, 4Department of

Infectious Diseases, University Medical Center Ljubljana, Ljubljana, Slovenia.

 

To delineate the inflammatory potential of the 3 pathogenic species of

Borrelia burgdorferi sensu lato, we stimulated monocyte-derived macrophages

from healthy human donors with 10 isolates each of B. burgdorferi, Borrelia

afzelii, or Borrelia garinii recovered from erythema migrans skin lesions of

patients with Lyme borreliosis from the United States or Slovenia. B.

burgdorferi isolates from the United States induced macrophages to secrete

significantly higher levels of interleukin (IL)-8, CCL3, CCL4, IL-6, IL-10, and

tumor necrosis factor than B. garinii or B. afzelii isolates. Consistent

with this response in cultured macrophages, chemokine and cytokine levels in

serum samples of patients from whom the isolates were obtained were

significantly greater in B. burgdorferi-infected patients than in B. afzelii- or

B. garinii-infected patients. These results demonstrate in vitro and in vivo

that B. burgdorferi has greater inflammatory potential than B. afzelii and

B. garinii, which may account in part for variations in the clinical

manifestations of Lyme borreliosis.

 

PMID: 19909078 [PubMed - as supplied by publisher]