Chrysanthemum Indicum Extract A Promising Treatment For Liver Cancer

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Chrysanthemum Indicum Extract A Promising Treatment For Liver Cancer

 

Chrysanthemum indicum Linné (Compositae) has long been used in traditional

Korean, Chinese, Japanese medicine to treat various immune-related diseases.

Chrysanthemum indicum extract (CIE) has been used in various studies with

promising results. A series of studies have demonstrated that Chrysanthemum

indicum possesses antimicrobial, antiinflammatory, immunomodulatory, and

neuroprotective effects. Recently, much attention has been devoted to the

anticancer activity of Chrysanthemum indicum, especially in hepatocellular

carcinoma (HCC).

 

The following study performed in rats with human cells showed CIE inhibited

proliferation of human heptocellular carcinoma (HCC) cells in a time- and

dose-dependent manner without cytotoxicity. CIE induced apoptosis which is cell

death of human HCC in a concentration-dependent manner, as determined by flow

cytometry. The cancer-specific selectivity shown in their study suggests that

the Chrysanthemum Indicum plant extract could be a promising novel treatment for

human cancer.

 

World J Gastroenterol. 2009 Sep 28;15(36):4538-46.

Induction of apoptosis and cell cycle arrest in human HCC MHCC97H cells with

Chrysanthemum indicum extract.

AIM: To investigate the effects of Chrysanthemum indicum extract (CIE) on

inhibition of proliferation and on apoptosis, and the underlying mechanisms, in

a human hepatocellular carcinoma (HCC) MHCC97H cell line. METHODS: Viable rat

hepatocytes and human endothelial ECV304 cells were examined by trypan blue

exclusion and MTT assay, respectively, as normal controls. The proliferation of

MHCC97H cells was determined by MTT assay. The cellular morphology of MHCC97H

cells was observed by phase contrast microscopy. Flow cytometry was performed to

analyze cell apoptosis with annexin V/propidium iodide (PI), mitochondrial

membrane potential with rhodamine 123 and cell cycle with PI in MHCC97H cells.

Apoptotic proteins such as cytochrome C, caspase-9, caspase-3 and cell cycle

proteins, including P21 and CDK4, were measured by Western blotting. RESULTS:

CIE inhibited proliferation of MHCC97H cells in a time- and dose-dependent

manner without cytotoxicity in rat hepatocytes and human endothelial cells. CIE

induced apoptosis of MHCC97H cells in a concentration-dependent manner, as

determined by flow cytometry. The apoptosis was accompanied by a decrease in

mitochondrial membrane potential, release of cytochrome C and activation of

caspase-9 and caspase-3. CIE arrested the cell cycle in the S phase by

increasing P21 and decreasing CDK4 protein expression. CONCLUSION: CIE exerted a

significant apoptotic effect through a mitochondrial pathway and arrested the

cell cycle by regulation of cell cycle-related proteins in MHCC97H cells without

an effect on normal cells. The cancer-specific selectivity shown in this study

suggests that the CI plant extract could be a promising novel treatment for

human cancer.

PMID: 19777612

 

This study showed CIE anti-inflammatory effects and examines the pathway.

J Ethnopharmacol. 2009 Apr 21;122(3):473-7. Epub 2009 Feb 3.

Chrysanthemum indicum Linné extract inhibits the inflammatory response by

suppressing NF-kappaB and MAPKs activation in lipopolysaccharide-induced RAW

264.7 macrophages.

PMID: 19429315

 

 

J Ethnopharmacol. 2005 Oct 3;101(1-3):334-7.

Anti-inflammatory and immunomodulatory activities of the extracts from the

inflorescence of Chrysanthemum indicum Linné.

PMID: 16029939

 

This research is still in the beginning phases and if you have liver cancer,

please consult with a doctor about your treatment.

 

Taken from:

http://www.healthyhepper.com/livercancerCIE.htm