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CHRONIC INFLAMMATION

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Aging and Inflammation

 

Chronic systemic inflammation is an underlying cause of many seemingly

unrelated, age-related diseases. As humans grow older, systemic inflammation

can

inflict devastating degenerative effects throughout the body (Ward 1995;

McCarty 1999; Brod 2000). This fact is often overlooked by the medical

establishment, yet persuasive scientific evidence exists that correcting a

chronic

inflammatory disorder will enable many of the infirmities of aging to be

prevented

or reversed.

 

The pathological consequences of inflammation are well documented in the

medical literature (Willard et al. 1999; Hogan et al. 2001). Regrettably, the

dangers of systemic inflammation continue to be ignored, even though proven

ways exist to reverse this process. By following specific prevention protocols

suggested by the Life Extension Foundation, the inflammatory cascade can be

significantly reduced.

 

The Causes of Age-Related Inflammation

 

Aging results in an increase of inflammatory cytokines (destructive

cell-signaling chemicals) that contribute to the progression of many

degenerative

diseases (Van der Meide et al. 1996; Licinio et al. 1999). Rheumatoid arthritis

is a classic autoimmune disorder in which excess levels of cytokines such as

tumor necrosis factor-alpha (TNF-a), interleukin-6 (IL-6), interleukin 1b

[iL-1(b)], and/or interleukin-8 (IL-8) are known to cause or contribute to the

inflammatory syndrome (Deon et al. 2001).

 

Chronic inflammation is also involved in diseases as diverse as

atherosclerosis, cancer, heart valve dysfunction, obesity, diabetes, congestive

heart

failure, digestive system diseases, and Alzheimer's disease (Brouqui et al.

1994; Devaux et al. 1997; De Keyser et al. 1998). In aged people with multiple

degenerative diseases, the inflammatory marker, C-reactive protein, is often

sharply elevated, indicating the presence of an underlying inflammatory

disorder (Invitti 2002; Lee et al. 2002; Santoro et al. 2002; Sitzer et al.

2002).

When a cytokine blood profile is conducted on people in a weakened condition,

an excess level of one or more of the inflammatory cytokines, e.g., TNF-a,

IL-6, IL-1(b), or IL-8, is usually found (Santoro et al. 2002).

 

Protecting Against Inflammatory-Related Disease

 

The New England Journal of Medicine published several studies in the year

2000 showing that the blood indicators of inflammation are strong predictive

factors for determining who will suffer a heart attack (Lindahl et al. 2000;

Packard et al. 2000; Rader 2000).

 

A growing consensus among scientists is that common disorders such as

atherosclerosis, colon cancer, and Alzheimer's disease are all caused in part by

a

chronic inflammatory syndrome.

 

Seemingly unrelated diseases have a common link. People who have multiple

degenerative disorders often exhibit excess levels of pro-inflammatory markers

in their blood. Here is a partial list of common medical conditions that are

associated with chronic inflammation:

 

 

Diseases Related To Chronic Inflammation Disease Mechanism Allergy

Inflammatory cytokines induce autoimmune reactions Alzheimer's Chronic

inflammation

destroys brain cells Anemia Inflammatory cytokines attack erythropoietin

production Aortic valve stenosis Chronic inflammation damages heart valves

Arthritis Inflammatory cytokines destroy joint cartilage and synovial fluid

Cancer Chronic inflammation causes many cancers Congestive heart failure

Chronic

inflammation contributes to heart muscle wasting Fibromyalgia Inflammatory

cytokines are elevated Fibrosis Inflammatory cytokines attack traumatized

tissue Heart attack Chronic inflammation contributes to coronary

atherosclerosis

Kidney failure Inflammatory cytokines restrict circulation and damage nephrons

Lupus Inflammatory cytokines induce an autoimmune attack Pancreatitis

Inflammatory cytokines induce pancreatic cell injury Psoriasis Inflammatory

cytokines induce dermatitis Stroke Chronic inflammation promoted thromboembolic

events Surgical complications Inflammatory cytokines prevent healing

 

 

A critical inflammatory marker is C-reactive protein. This marker indicates

an increased risk for destabilized atherosclerotic plaque and abnormal

arterial clotting. When arterial plaque becomes destabilized, it can burst open

and

block the flow of blood through a coronary artery, resulting in an acute

heart attack. One of the New England Journal of Medicine studies showed that

people with high levels of C-reactive protein were almost three times as likely

to die from a heart attack (Ridker et al. 1997).

 

The Life Extension Foundation long ago advised members to have an annual

C-reactive protein blood test to detect systemic inflammation that could

increase the risk of heart attack, stroke, cancer and a host of age-related

diseases. In fact, on January 28, 2003, the American Heart Association and

Centers

for Disease Control & Prevention (CDC) jointly endorsed the C-reactive protein

test to screen for coronary-artery inflammation to identify those at risk for

heart attack.

 

What Causes Elevated C-reactive Protein?

* Elevated C-Reactive Protein and Interleukin-6 Predict Type II

Diabetes

While some doctors are finally catching on to the fact that elevated

C-reactive protein increases heart attack and stroke risk, they still know

little

about its other dangers. Even fewer practicing physicians understand that

pro-inflammatory cytokines are an underlying cause of systemic inflammation that

is indicated by excess C-reactive protein in the blood.

 

In an abstract published in the March 6, 2002 issue of the Journal of the

American College of Cardiology (JACC), tumor necrosis factor-alpha (TNF-a)

levels were measured in a group of people with high blood pressure and a group

with normal blood pressure (Verdeccnia et al. 2002). The objective of this

study was to ascertain if arterial flow mediated dilation was affected by

hypertension and chronic inflammation as evidenced by high levels of the

pro-inflammatory cytokine TNF-a.

 

The hypertensive subjects taking anti-hypertensive medications had about the

same blood pressure as the healthy test subjects. Arterial flow medicated

dilation, however, was significantly impaired in the hypertensives and this

group also showed higher levels of TNF-a, indicating persistent inflammation

despite blood pressure control. This study showed that even when blood pressure

is under control, hypertensives still suffer from continuous damage to the

inner lining of the arterial wall (endothelial dysfunction) caused by a chronic

inflammatory insult. The doctors who conducted this study concluded by

stating:

 

" Antihypertensive therapy alone may be insufficient to improve endothelial

dysfunction in hypertensives with high plasma levels of inflammatory markers.

Additional therapy to target inflammation may be necessary to improve

endothelial function and to prevent progression of coronary atherosclerosis in

high-risk hypertensives with subclinical inflammations. "

A sensitive index to evaluate how much endothelial damage is occurring is

the measurement of TPA (tissue-type plasminogen activator), a clot-dissolving

enzyme found in the blood. This same study showed elevated TPA levels in

hypertensives, indicating continued endothelial damage despite blood pressure

reduction. These findings indicate that hypertensives should have their blood

tested for both TNF-a and TPA to assess how much inner wall (endothelial)

arterial damage is occurring (Vardecchia et al. 2002). If TNF-a and/or TPA

levels

are high, aggressive therapies to suppress the inflammatory cascade should be

considered.

 

Elevated C-Reactive Protein and Interleukin-6 Predict Type II Diabetes

 

In a study published in the July 18, 2001 issue of the Journal of the

American Medical Association, a group from the famous Women's Health Study were

evaluated to ascertain what risk factors could predict future development of

Type II diabetes (Prudhan et al. 2001). The findings showed that baseline

levels

of C-reactive protein and interleukin-6 (IL-6) were significantly higher

among those who subsequently developed diabetes compared to those who did not.

 

When comparing the highest versus lowest quartile, women with the higher

IL-6 levels were 7.5 times more likely to develop diabetes while those in the

higher C-reactive protein ranges were 15.7 times more likely to become

diabetic. After adjusting for all other known risk factors, women with the

highest

IL-6 levels were 2.3 times at greater risk, while those with the highest

C-reactive protein levels were 4.2 times more likely to become diabetic. It

should

be noted that these other diabetic risk factors (such as obesity, estrogen

replacement therapy and smoking) all sharply increase inflammatory markers in

the blood. The doctors who conducted this study concluded by stating:

 

" Elevated C-reactive protein and IL-6 predict the development of Type II

diabetes mellitus. These data support a possible role for inflammation in

diabetogenesis. "

 

C-Reactive Protein and IL-6 Predict Death

* Elderly Frailty

* Glycation's Role

* Cooking and Aging

* Diet

It is well established the elevated C-reactive protein, IL-6 and other

inflammatory cytokines indicate significantly greater risks of contracting or

dying from specific diseases (heart attack, stroke, Alzheimer's disease, etc.).

 

A group of doctors wanted to ascertain if C-reactive protein and IL-6 could

also predict the risks of all-cause mortality. In a study published in the

American Journal of Medicine, a sample of 1,293 healthy elderly people were

followed for a period of 4.6 years (Harris et al. 1999). Higher IL-6 levels

were

associated with a twofold greater risk of death. Higher C-reactive protein

was also associated with a greater risk of death, but to a lesser extent than

elevated IL-6. Subjects with both high C-reactive protein and IL-6 were 2.6

times more likely to die during follow up than those with low levels of both

of these measurements of inflammation. These results were independent of all

other mortality risk factors. The doctors concluded by stating:

" These measurements (C-reactive protein and IL-6) may be useful for

identification of high-risk subgroups for anti-inflammatory interventions. "

Frailty in Elderly Linked to Inflammation

 

In a study of almost 5,000 elderly people, scientists discovered that frail

seniors were more likely to have signs of increased inflammation than their

more active counterparts. This study was published in the Archives of Internal

Medicine (Walston et al. 2002) and showed that these frail seniors with

elevated blood inflammatory markers also tended to show more clotting activity,

muscle weakness, fatigue and disability than active elderly people.

 

Findings from these studies should motivate every health conscious

individual to have their blood tested for C-reactive protein. If it is

elevated, then

the Inflammatory Cytokine Test Panel is highly recommended. Those who suffer

from any type of chronic disease may also consider the Inflammatory Cytokine

Test Panel in order to identify the specific inflammatory mediator that is

causing or contributing to their problem.

 

Glycation's Role in Inflammation

 

Eating high temperature cooked food is another contributor in the production

of inflammatory cytokines. In fact, it has been shown that eating high

temperature cooked food leads to the formation of advanced glycation end (AGE)

products. Glycation can be described as the binding of a protein molecule to a

glucose molecule resulting in the formation of damaged protein structures.

Many age-related diseases such as arterial stiffening, cataract and

neurological impairment are at least partially attributable to glycation. These

destructive glycation reactions render proteins in the body cross-linked and

barely

functional. As these degraded proteins accumulate, they cause cells to emit

signals that induce the production of inflammatory cytokines.

 

The glycation process is presently irreversible, though an important study

indicates a drug in clinical trials may be partially effective. According to a

Proceedings of the National Academy of Sciences study, consuming foods

cooked at high temperature accelerates the glycation process, and the

subsequent

formation of advanced glycation end products.

 

A more succinct descriptive term for " advanced glycation end products " is

" glycotoxin, " since " advanced glycation end products " are toxic to the body. We

will use the word " glycotoxin " from here on to describe the term " advanced

glycation end products. "

 

Cooking and Aging Have Similar Biological Properties

 

Cooking foods at high temperatures results in a " browning " effect, where

sugars and certain oxidized fats react with proteins to form glycotoxins in the

food. Normal aging can also be regarded as a slow cooking process, since

these same glycotoxins form in the skin, arteries, eye lenses, joints,

cartilage,

etc. of our body.

 

The Proceedings of the National Academy of Sciences study shows that

consuming foods high in glycotoxins might be responsible for the induction of a

low-grade, but chronic state of inflammation. In addition, the glycotoxins in

food cooked at high temperatures also promote the formation of glycotoxins in

our living tissues. The implication of these findings is profound.

 

Diet and Inflammation

 

What one eats plays a major role in chronic inflammatory processes.

Consuming low glycemic foods prevents the insulin surge that contributes to

chronic

inflammatory processes. It is also important to avoid over consumption of

foods high in arachidonic acid (beef, egg yolk, dairy, etc.).

 

We now know that eating too much over-cooked food causes an increase in

inflammatory cytokines. Since most " junk " foods are cooked at extremely high

temperatures, it makes sense to avoid French fries, hamburgers, potato chips,

fried food and other snacks. These foods not only contain lots of glycotoxins,

they also create other metabolic disorders that can induce degenerative

disease.

 

Consuming at least 1000 mg a day of carnosine, and/or 300 mg of the European

drug aminoguanidine can inhibit pathological glycation reactions in the b

ody. Eating high temperature cooked foods also induces the formation of

glycotoxins. Avoiding foods cooked at high temperature not only reduces

pathological

glycation processes, but also prevents the formation of numerous

gene-mutating toxins that are known carcinogens.

 

Food is cooked to destroy bacteria and other pathogens that could cause a

serious illness. It is important not to eat undercooked food, but avoiding food

unnecessarily cooked at higher temperatures is desirable. Certain foods

(like fried foods) have to cook at high temperatures. Health conscious people

are

increasingly avoiding fried foods because they are associated with many

health risks.

 

With the availability of cytokine blood profile tests, it is now possible to

ascertain the underlying cause of chronic inflammatory disease. The

appropriate drugs, nutrients, dietary change(s) and/or hormones can then be

used to

suppress the specific cytokines (such as IL-6 or TNF-a) that are promoting

the inflammatory cascade.

 

The Detrimental Effects of Sleep Deprivation

 

On June 22, 2002, researchers at the annual meeting of the Endocrine Society

held in San Francisco reported that sleep deprivation markedly increases

inflammatory cytokines. This finding helps explain why pain flare-up occurs in

response to lack of sleep in a variety of disorders. According to the

researchers, even modest sleep restriction adversely affects hormone and

cytokine

levels. In this carefully controlled study, sleep deprivation caused a 40% to

60% average increase in the inflammatory marker IL-6 in men and women, while

men alone showed a 20% to 30% increase in TNF-a. Both IL-6 and TNF are potent

pro-inflammatory cytokines that induce systemic inflammation (Vgontzas et al.

1999; Vgontzas et al. 2001).

 

The study results were presented by Dr. Alexandros Vgontzas, professor of

psychiatry at The Pennsylvania State University in Hershey. Dr. Vgontzas stated

that the findings indicate that getting a full night's rest of eight hours

is not just a nice bonus, but a necessity. He stated that people who are

missing even two to three hours of sleep function poorly the next day.

 

Dr. Vgontzas added that the finding that lack of sleep may stimulate an

increase in chronic inflammatory response is worrisome because inflammation has

been linked to the most common lethal conditions affecting humans today.

Vgontzas warned: " Restriction of sleep a few hours is a major risk for public

safety. "

 

This study has significant implications for the treatment of chronic pain

and inflammatory disorders.

 

The Dangerous Pro-Inflammatory Cytokines

* Reduction

* Lowering Elevated C-Reactive Protein

* Cytokine Blood Testing

* Importance of Cytokine Testing

* Pentoxifylline Studies

* Diet

The following acronyms represent the most dangerous pro-inflammatory

cytokines. Health-conscious persons should become familiar with these terms

because

excess levels of these cytokines cause or contribute to many diseases states:

 

* TNF-a tumor necrosis factor-alpha

* IL-6 interleukin-6

* IL-1(b) interleukin-1 beta

* IL-8 interleukin-6

Reducing Inflammation

 

Scientists have identified dietary supplements and prescription drugs that

can reduce levels of the pro-inflammatory cytokines. The docosahexaenoic acid

(DHA) fraction of fish oil is the best documented supplement to suppress

TNF-a, IL-6, IL-1(b), and IL-8 (Jeyarajah et al. 1999; James et al. 2000;

Watanabe et al. 2000; Yano et al. 2000). A study on healthy humans and those

with

rheumatoid disease shows that fish oil suppresses these dangerous cytokines by

up to 90% (James et al. 2000).

 

Other cytokine-lowering supplements are DHEA (Casson et al. 1993), vitamin K

(Reddi et al. 1995; Weber 1997), GLA (gamma linolenic acid) (Purasiri et al.

1994), and nettle leaf extract (Teucher et al. 1996). Antioxidants, such as

vitamin E (Devaraj et al. 2000) and N-acetyl-cysteine (Gosset et al. 1999),

may also lower proinflammatory cytokines and protect against their toxic

effects.

 

Prescription drugs like Enbrel ($10,000 a year) directly bind to TNF-a and

block its interaction with TNF cell surface receptors. Enbrel has demonstrated

significant clinical improvement in rheumatoid arthritis patients, as have

high-dose fish oil supplements (Kremer 2000). High levels of TNF-a may persist

even in people receiving Enbrel drug therapy. Even if Enbrel brings TNF-a

down to a safe range, other inflammatory cytokines such as IL-6 and IL-1(b) may

continue to wreak havoc throughout the body. High levels of tumor necrosis

factor (TNF-a) are destructive to many vital tissues such as joint cartilage

(e.g., rheumatoid arthritis) and heart muscle (e.g., congestive heart failure).

 

Excess IL-6 and other inflammatory cytokines attack bone and promote the for

mation of fibrinogen that can induce a heart attack or stroke (di Minno et

al. 1992). To prevent and treat the multiple diseases of aging, it is critical

to keep these destructive immune chemicals (cytokines) in safe ranges.

 

Methods of Lowering Elevated C-Reactive Protein

 

Those who are in relative good health, but have elevated C-reactive protein,

can try to lower it using a variety of diet modifications, supplements

and/or drugs. Supplements such as vitamin E, borage oil, fish oil, DHEA,

vitamin K

and nettle leaf extract can lower C-reactive protein. Diets low in

arachidonic acid, omega-6 fatty acids, saturated fats, high-glycemic food and

overcooked food can suppress inflammatory factors in the body.

 

If diet and supplements fail, drugs such as ibuprofen, aspirin,

pentoxifylline or one of the statins (such as Pravachol®) should be tried. If

the

modified diet, nutrients and/or drugs lower C-reactive protein to below 1.3

(mg/L)

of blood, then this is an indication that the underlying inflammatory fire has

been extinguished. (The high-sensitivity C-reactive protein blood test is

recommended to measure this indicator.)

 

For those whose blood tests reveal persistently high inflammatory cytokine

levels despite taking the supplements mentioned above, a low-cost prescription

drug may be of enormous benefit.

 

The generic name of this low-cost prescription drug is pentoxifylline (PTX);

the brand name is Trental. This drug was first used in Europe in 1972 and

long ago was removed from patent status (meaning it is not cost-prohibitive).

PTX is prescribed to improve blood flow properties by decreasing its

viscosity. It works by improving red blood cell flexibility, decreasing

platelet

aggregation, and reducing fibrinogen levels (de la Cruz et al 1993; Gara 1993;

Gaur et al. 1993). PTX has fallen from favor because no drug company has the

economic incentive to market it to physicians. PTX is primarily prescribed to

patients with peripheral artery disease, although it may have potential

efficacy in treating a wide range of diseases relating to chronic inflammation.

 

Numerous studies show that pentoxifylline (PTX) is a potent inhibitor of

TNF-a, IL-1(b), IL-6, and other pro-inflammatory cytokines (Neuner et al. 1994;

Noel et al. 2000; Pollice et al. 2001; Ventura et al. 2001). Similarly,

studies also show that DHA fish oil suppresses these same cytokines (Das 2000;

Yano et al. 2000). In people who have a chronic disease involving elevated

levels of the inflammatory cytokines, the daily administration of 400-800 mg of

PTX and/or 1000-2000 mg of DHA fish oil could be of enormous benefit.

 

Individuals with chronic disease sometimes find it difficult to suppress

C-reactive protein. In these cases, it is important to identify the specific

inflammatory cytokines that are responsible for the destructive inflammatory

processes that is causing or contributing to the underlying disease state. This

enables a custom tailored program to be implemented, and its success measured

by suppressing the pro-inflammatory cytokine culprits. For instance, if

levels of TNF-a levels are elevated, and natural approaches fail to lower it,

the

prescription drug Enbrel should be considered.

 

Inflammatory Cytokine Blood Testing

 

People suffering from chronic disease often have elevated levels of

C-reactive protein in their blood. C-reactive protein indicates an inflammatory

process is going on in the body, but does not identify the specific

pro-inflammatory cytokine that may be the underlying cause.

 

Below is the cytokine panel for this test along with the optimal

anti-inflammatory ranges:

 

Pro-inflammatory Cytokine Optimal Anti-Inflammatory Range Quest LabCorp

Tumor necrosis factor alpha (TNF-a) 0-25 pg/mL < 8.1 pg/mL Interleukin-1 beta

(IL-1b) 0-150 pg/mL < 15.0 pg/mL Interleukin-6 (IL-6) 2-29 pg/mL < 12.0 pg/mL

Interleukin-8 (IL-8) 10-80 pg/mL < 32.0 pg/mL

 

Note: Quest and LabCorp are blood testing facilities. Other blood testing

laboratory methods may have different ranges.

 

As stated earlier in this chapter, an inexpensive C-reactive protein

(high-sensitivity) blood test (CRP-hs) can help reveal if you have systemic

inflammation. If your C-reactive protein level is over 1.3 (mg/L), this is an

indication that you have an inflammatory event occurring in your body. Those

with

elevated CRP-hs levels (and who have a disease associated with chronic

inflammation) should consider using a supplement protocol and/or prescription

drugs

known to suppress elevated pro-inflammatory cytokines.

 

The Importance of Cytokine Testing for Those Suffering From Chronic Illness

 

There are many chronic disease states that can now be managed by the proper

utilization of the Inflammatory Cytokine Blood Panel. If you are elderly, or

suffer from any serious disorder, these cytokine tests can enable your doctor

to prescribe therapies that specifically target the inflammatory cytokine

responsible for your poor state of health.

 

From a practical standpoint, if you suffer from congestive heart failure,

and your levels of TNF-a remain persistently high, you may ask your doctor to

prescribe the drug EnbrelÆ, which specifically counteracts the destructive

effects of TNF-a.

 

If you suffer from cancer and your levels of IL-6 remain persistently high,

you may consider high dose DHEA or asking your doctor to prescribe a

bisphosphonate drug (such as ZometaÆ that protects against bone destruction

that

releases excess IL-6 into the body. Those with prostate, certain types of

breast

cancer, and other hormonally driven cancer should consider other IL-6

lowering therapies (such as high dose DHA fish oil extract) in lieu of DHEA.

 

Some cancer and patients display elevated levels of IL-8, which induces

cancer cells to express growth factors that fuel their propagation. In

hepatitis

C, elevated IL-8 signals interferon drug resistance. An IL-8 suppressing

therapy will soon be available to Americans (it is already used in Japan).

 

Those with systemic inflammatory disease often manifest high levels of

IL-1b. If diet, the anti-inflammatory supplements (fish oil, borage oil, DHEA,

etc.) and cytokine-suppressing drugs (pentoxifylline, 400 mg twice a day) fail

to suppress this destructive cytokine, then ask your doctor to prescribe the

drug Arava (leflunomide), starting at the low dose of 10 mg a day.

 

Pentoxifylline Studies

 

This section discusses the positive results obtained in numerous studies

when pentoxifylline was administered to reduce the damaging effects of chronic

inflammation.

 

PTX is a prescription drug approved by the FDA to treat peripheral vascular

disease. The standard dose is 1200 mg daily to improve circulation. To

suppress pro-inflammatory cytokines, a lower dose of 400 mg twice a day can be

used. A brief description of studies showing benefits of PTX extending beyond

its

FDA-approved use follows.

 

A controlled study on human diabetics with advanced renal failure showed

that 400 mg daily of PTX reduced TNF-a levels by approximately 35%. In the PTX

group, a measurement of kidney impairment was reduced 59%. There were no

changes in those given placebo. The researchers noted that inflammatory

cytokines

such as TNF-a have long been implicated in the development and progression

of diabetic kidney failure (Navarro et al. 1999a). Organ failure induced by

TNF-a has also been confirmed by other studies (Boldt et al. 2001).

 

Aging causes a progressive decline of blood delivery to the tissues. Those

who have diabetes experience accelerated circulatory deficit. In a study on

diabetic rats, just 2 weeks of PTX administration resulted in a correction of

nerve conduction deficit, amounting to 56.5% in the sciatic motor nerve and

69.8% in the saphenous sensory nerve. PTX restored the microvascular deficit by

50.4% (Flint et al. 2000). This study indicates that PTX may be of

particular benefit to diabetics, especially those suffering from neuropathy,

kidney

disease, and other vascular disorders.

 

It is not just age-related disease that has been linked to chronic

inflammation. A growing body of evidence points to a chronic inflammatory state

as an

underlying cause of kidney failure, asthma, pancreatitis, lupus, certain skin

diseases, and other conditions.

 

In a study on human asthmatics (Entzian et al. 1998), PTX was shown to be

almost 6 times more effective in suppressing TNF-a than the popular anti-asthma

drug theophylline. The doctors concluded that PTX may be an especially

promising candidate as an asthma therapy.

 

Lupus is an autoimmune disease. About 90% of its victims develop kidney

problems. In a group of pediatric lupus patients, PTX helped to stop the

deterioration of kidney function (Vazquez Garcia et al. 2000). The clinical

manifestations of experimental systemic lupus erythematosus (SLE) correlate

with an

increased secretion of TNF-a and IL-1(b). In a mouse study, PTX significantly

reduced the production of IL-1b and TNF-a. The result was significantly lower

anti-DNA antibodies (a blood marker of lupus activity) and a substantially

lower rate of protein in the urine (indicating reduced kidney damage). The

scientists concluded that the early administration of PTX improves the clinical

status of mice with this autoimmune disease (lupus) (Segal et al. 2001).

 

In advanced kidney failure, anemia can be induced by an inflammatory

cytokine attack on erythropoietin, the major natural hormone responsible for

red

blood cell (RBC) production. In a group of seven anemic patients with advanced

renal failure, PTX suppressed TNF-a and reversed the anemic state (Navarro et

al. 1999b).

 

Free radicals and inflammatory cytokines have been implicated in

pancreatitis. Inflammation of the pancreas is associated with a greater risk of

pancreatic cancer. Many of the antioxidants used by Foundation members reduce

the

incidence of pancreatitis. In one study on acute pancreatitis, PTX was shown to

reduce pancreatic inflammation and attenuate the depletion of pancreatic

glutathione. PTX also inhibited the expected increase in TNF-a levels and

prevented mitochondrial damage. Mitochondria are the power plants within all of

our

cells. The scientists suggested that PTX be considered as an adjuvant

treatment of acute pancreatitis (Gomez-Cambronero et al. 2000).

 

Psoriasis is characterized by abnormal cell proliferation, inflammation, and

increased levels of inflammatory cytokines. In an experiment on nude mice,

PTX was shown to reduce cell proliferation and thickening of skin. Improvement

was seen in the classical signs of psoriasis (Gilhar et al. 1996). A study

on dogs showed that PTX was one of several drugs helpful in treating atopic

dermatitis (Marsella et al. 2001). In mice, a study showed PTX to be effective

in treating contact- and irritant-induced dermatitis by suppressing excess

production of TNF-a (Schwarz et al. 1993).

 

An increase in TNF-a has been implicated in leprosy skin reactions. PTX has

also been shown to work with other drugs in producing a quick response to

this inflammatory cytokine-induced condition (Sampaio et al. 1998; Welsh et al.

1999).

 

Fibrosis is a common problem for cancer patients undergoing radiation

therapy. PTX in combination with vitamin E has been shown to help heal these

lesions. Scientists have speculated that the efficacy of this treatment is

probably

due to a combination of blood flow stimulation and reduction in inflammatory

cytokines (Fischer et al. 2001). Other studies show that PTX helps to

prevent the fibrosis (Moser et al. 2000).

 

Inflammation plays a pivotal role in the pathogenesis of organ injury after

cardiopulmonary bypass. Elderly patients appear to be especially prone to

developing systemic inflammation. In a controlled study, patients undergoing

cardiopulmonary bypass were given PTX before and right after surgery. Compared

to the group receiving PTX, the control group showed a greater increase in

C-reactive protein, IL-6, and other inflammatory cytokines. The PTX-treated

patients recovered faster than the controls (Boldt et al. 2001). The

researchers

conducting the study stated the PTX group showed less inflammatory response

than the controls and urged that more studies be done.

 

When it comes to healing after surgery, several factors are involved

including restoration of microcirculation and strength of the inflammatory

response.

In a study on rats, PTX significantly shortened the time needed for healing

in colonic anastomoses (reconnecting the large intestine after removing a

section of it as occurs for colon cancer patients). In the rats receiving PTX,

inflammatory response was markedly reduced and restoration of circulation

improved. The scientists concluded by stating that PTX administration could

prevent failures of colonic anastomoses (Schwarz et al. 1993). This study

provides

further evidence that PTX can be of significant benefit to the surgical

patient by speeding the healing process. High DHA fish oil may also provide

these

benefits.

 

Some surgeons might be concerned that PTX could cause excess bleeding, yet

one study showed that by modulating the dose of various anti-clotting agents

(including PTX), the risk of surgical bleeding and abnormal blood clots could

be reduced (Schwarz et al. 1993). The real value to PTX may be its long-term

use after surgery to protect against the chronic inflammatory syndrome, to

which so many of the elderly are vulnerable. The maintenance dose of PTX needed

may be as low as 400 mg daily. (Remember: High-dose fish oil and other

nutrients have shown similar benefits to PTX.)

 

When to Avoid PTX and Other Anti-Inflammatories. PTX should not be used by

individuals with bleeding disorders such as a recent cerebral or retinal

hemorrhage (PDR 2001). Patients taking Coumadin should have more frequent

monitoring (once a week) of prothrombin times (White et al. 1989; Stigendal et

al.

1999). Those with other types of bleeding should receive frequent physician

examinations. According to two studies, PTX should be avoided by Parkinson's

disease patients (Godwin-Austen et al. 1980; Serrano-Duenas et al. 2001).

 

It is important to note that the body uses TNF-a to acutely fight

infections. If patients show any sign of infectious disease, drugs such as

Enbrel (that

inhibit the effects of TNF-a), are temporarily discontinued. A new FDA

advisory states that patients should be tested and treated for inactive

tuberculosis prior to therapy with another TNF-a inhibiting therapy

(infliximab).

Because PTX, fish oil, and nettle directly suppress TNF-a, these agents should

be

temporarily discontinued during the time when one has an active infection.

 

Sources of Pentoxifylline. Pentoxifylline can be obtained from any pharmacy

with a physician's prescription. Because only 1-2 tablets daily are taken,

pentoxifylline is a relatively inexpensive drug.

 

Diet and Inflammation

 

In addition to toxic cytokines, there are other inflammatory pathways that

can be mediated via diet modification. A common problem involves

overproduction of proinflammatory hormone-like " messengers " (such as

prostaglandin E2) and

underproduction of anti-inflammatory " messengers " (such as prostaglandin E1

and E3).

 

The good news is that omega-3 fatty acids found in fish oil help to suppress

the formation of undesirable prostaglandin E2 and promote synthesis of

beneficial prostaglandin E3 (Kelley et al. 1985; Watanabe et al. 2000). Gamma

linolenic acid (GLA) induces production of the anti-inflammatory prostaglandin

E1

(Das et al. 1989; Fan et al. 1997). What you eat can significantly affect

whether you have more of the beneficial prostaglandins (E1 and E3) as opposed

to the pro-inflammatory prostaglandin E2.

 

Because prostaglandin E2 is a culprit in inflammation, reducing the

consumption of foods that are high in omega-6 fatty acids and increasing the

consumption of omega-3 rich foods, such as salmon and other fish, can be

beneficial.

Limiting foods that convert to arachidonic acid can help reduce inflammation.

Arachidonic acid is a precursor to both prostaglandin E2 and the

pro-inflammatory cytokine leukotriene B(4) (Brock et al. 1999). Another dietary

factor

that can lead to high levels of arachidonic acid is the overconsumption of

high-glycemic index carbohydrates that cause excess production of insulin

(Kreisberg et al. 1983). These quickly digestible foods include fruit juices or

rice cakes. Food heavy in polyunsaturated fats or saturated fats can also

increase prostaglandin E2.

 

Additionally, a study of elderly patients with heart disease requiring

elective surgery (Tepaske et al. 2001) found that nutritional supplements

containing omega-3 polyunsaturated fatty acids (as well as yeast and

L-arginine)

improved the outlook for high-risk patients when given a minimum of 5 days

prior

to surgery.

 

SUMMARY

 

The number of inflammatory-related diseases that could be successfully

treated with cytokine-lowering therapy is staggering. PTX and supplements such

as

fish oil, nettle leaf, DHEA, and vitamin K possess mechanisms of suppressing

inflammatory cytokines. Unfortunately, there are no side-by-side comparisons

to enable us to categorically state whether PTX or natural agents (such as

DHA fish oil) work better.

 

Foods cooked at high temperatures can produce a browning effect in which

glycotoxins are formed from the reaction of sugars and oxidized fats with

protein. Glycotoxins may contribute to low-grade chronic inflammation. High

glycemic foods may also contribute to the inflammatory process. Dietary

modifications to reduce inflammation should include elimination of foods and

cooking

processes that contribute to a chronic state.

 

For those who have multiple degenerative diseases, the cytokine profile

blood test and the C-reactive protein blood test are highly recommended. This

may

be done through your own physician or the Life Extension Foundation. If your

cytokine test reveals excess levels of cytokines such as TNF-a, IL-1(b), or

both, nutritional supplementation, dietary modifications, and low-cost

prescription medications such as PTX are advised.

 

The following supplements are suggested:

1. The docosahexaenoic acid (DHA) fraction of fish oil may be the most

effective nonprescription supplement to suppress pro-inflammatory cytokines.

Gamma linolenic acid (GLA) is a precursor of PGE1, a potent anti-inflammatory

agent. A product called _Super GLA/DHA_

(http://www.lifeextensionvitamins.com/gladha.html) provides 920 mg of GLA,

1000 mg of DHA, and 400 mg of EPA in 6

capsules.

2. _DHEA_ (http://www.lifeextensionvitamins.com/dhea15mg.html) is a

hormone that decreases with age. DHEA has been shown to suppress IL-6, an

inflammatory cytokine that often increases as people age. Typical doses of DHEA

are 25-50 mg daily, although some people take 100 mg daily. Refer to the _DHEA

Replacement protocol_ (http://www.lifeextensionvitamins.com/dhreth.html) for

suggested blood tests to safely and optimally use DHEA.

3. _Nettle leaf_ (http://www.lifeextensionvitamins.com/nettles.html)

has been shown to suppress the pro-inflammatory cytokine TNF-a. Take 1000 mg

daily.

4. Vitamin E and _N-acetyl-cysteine (NAC)_

(http://www.lifeextensionvitamins.com/naccys.html) are protective antioxidants

with anti-inflammatory

properties. Vitamin E that contains gamma tocopherol and tocotrienols provides

the most broad-spectrum protection. Take 1-2 capsules daily of _Gamma E

Tocopherols/Tocotrienols._

(http://www.lifeextensionvitamins.com/gaewitotolop.html)

NAC is an amino acid with antiviral and liver protectant properties. One

600-mg capsule daily is recommended.

5. _Vitamin K_ (http://www.lifeextensionvitamins.com/supervitamink.html)

helps reduce levels of IL-6, a pro-inflammatory messenger. Vitamin K also

helps in the treatment of osteoporosis by regulating calcium and promoting

bone calcification. One 10-mg capsule daily is recommended for prevention

purposes.

6. Consuming at least 1000 mg a day of _carnosine_

(http://www.lifeextensionvitamins.com/carnosine.html) and/or 300 mg of the

European drug

aminoguanidine can inhibit pathological glycation reactions in the body.

Note: It is illegal for the manufacturers of PTX to distribute this

off-label information to the public. Life Extension can provide this

information

because it does not sell PTX.

 

 

 

 

 

 

 

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