Thanks! Emmanuel

[Guest guest]

You are so helpful! I'm going to do more searching as you did and then

forward all the information to my friend. He'll be very glad to get

them. Thanks again!

 

Best regards!

 

You Chen

 

, " Emmanuel Segmen "

<susegmen@i...> wrote:

> Dear You Chen,

>

> Here's a few more papers for your friend to peruse. He won't have

to leave China to work in these labs.

>

> All the Best,

> Emmanuel Segmen

>

> [sequence analysis of 25S rDNA from Chinese medicinal plant

duzhong Eucommia ulmoides Oliv.]

> Zhongguo Zhong Yao Za Zhi 1998 Dec;23(12):707-9, 762 (ISSN:

1001-5302)

> Chen Y; Qu L; Zhou H; Zhang H

> School of Life Science, Zhongshan University, Guangzhou 510275.

> OBJECTIVE: To provide new molecular data for the

identification of the Chinese medicinal plant Duzhong (Eucommia

ulmoides). METHOD: Sequencing and analyzing the 25S rDNA gene(rDNA) 5'

terminal region. RESULT: More than 300 nucleotides of 25S rDNA 5'

terminal region were determined, and the specific nucleotides can be

used for the identification of this medicinal plant. CONCLUSION: This

sequence analysis may serve as a basis for preparing the specific rDNA

probe.

> _____

>

>

> [Chemical pattern recognition of traditional Chinese medicine

kudingcha (I)]

> Zhong Yao Cai 1998 Mar;21(3):115-9 (ISSN: 1001-4454)

> Su W; Wu Z; Chen J; He X; Li J

> Guangdong College of Pharmacy, Guangzhou 510224.

> In this paper, the non-linear mapping method of pattern

recognition was adopted to classify 78 samples of traditional Chinese

medicine Kudingcha, with macro and trace elements as classified

characteristic features. Ilex cornuta Lindl., Ilex latifolia Thunb.

and Ligustrum lucidum Ait. were identified accurately. The results

agree with those from pharmacognosy. This paper provides a new method

for identification of traditional Chinese medicine.

>

>

> ________

>

> Sequence-specific electrochemical detection of asymmetric PCR

amplicons of traditional Chinese medicinal plant DNA.

> Anal Chem 2002 Oct 1;74(19):5057-62 (ISSN: 0003-2700)

> Lee TM; Hsing IM

> Department of Chemical Engineering, The Hong Kong University

of Science and Technology, Clear Water Bay, Kowloon.

> In this study, an electrochemistry-based approach to detect

nucleic acid amplification products of Chinese herbal genes is

reported. Using asymmetric polymerase chain reaction and

electrochemical techniques, single-stranded target amplicons are

produced from trace amounts of DNA sample and sequence-specific

electrochemical detection based on the direct hybridization of the

crude amplicon mix and immobilized DNA probe can be achieved.

Electrochemically active intercalator Hoechst 33258 is bound to the

double-stranded duplex formed by the target amplicon hybridized with

the 5'-thiol-derivated DNA probe (16-mer) on the gold electrode

surface. The electrochemical current signal of the hybridization event

is measured by linear sweep voltammetry, the response of which can be

used to differentiate the sequence complementarities of the target

amplicons. To improve the reproducibility and sensitivity of the

current signal, issues such as electrode surface cleaning, probe

immobilization, and target hybridization are addressed. Factors

affecting hybridization efficiency including the length and binding

region of the target amplicon are discussed. Using our approach,

differentiation of Chinese herbal species Fritillaria (F. thunbergii

and F. cirrhosa) based on the 16-mer unique sequences in the spacer

region of the 5S-rRNA is demonstrated. The ability to detect PCR

products using a nonoptical electrochemical detection technique is an

important step toward the realization of portable biomicrodevices for

on-spot bacterial and viral detections.