Erythrina poeppigiana isoflavonoid & Staphylococcus aureus

[Guest guest]

Hi Holger,

 

See this:

 

Tanaka, H.; Sato, M.; Oh-Uchi, T.; Yamaguchi, R.; Etoh, H.; Shimizu, H.;

Sako, M.; Takeuchi, H. Antibacterial properties of a new isoflavonoid from

Erythrina poeppigiana against methicillin-resistant Staphylococcus aureus.

Phytomedicine 2004, 11 (4), 331-7. Dept Natural Product Chemistry, Faculty of

Pharmacy, Meijo University, Yagoto, Tempaku-ku, Nagoya, Japan. A new

isoflavonoid, together with four known isoflavonoids, was isolated from the

roots

of Erythrina poeppigiana. The chemical structure was determined by extensive

spectroscopic studies, and then its antibacterial activity against methicillin-

resistant Staphylococcus aureus (MRSA) was investigated. The new

isoflavonoid was identified as 3,9-dihyroxy-10-gamma,gamma-dimethylallyl-

6a,11a-dehydropterocarpan (compound 1). Compound 1 inhibited bacterial

growth most potently of the five isolates, and had a minimum inhibitory

concentration (MIC) of 125 microg/ml against thirteen MRSA strains. Inhibitory

activity was based on bactericidal action and viable cell number reduced by

approximately 1/10,000 after 4 h incubation with compound 1. Despite intense

bactericidal action against MRSA, compound 1 never resulted in leakage of 260

nm-absorbing substances from bacterial cells. Compound 1 (12.5 microg/ml)

completely inhibited incorporation of radio-labeled thymidine, uridine and

leucine into MRSA cells. Although glucose incorporation was also markedly

inhibited by the compound, the amount of glucose incorporated by bacterial

cells increased gradually with incubation time. These

findings suggest that

compound 1 exhibits anti-MRSA activity by interfering with incorporation of

metabolites and nutrients into bacterial cells or by affecting the nucleic acids

of

MRSA cells. Furthermore, this new compound could be a potent

phytotherapeutic agent for treating MRSA infections.

 

 

 

Best regards,

 

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