jiao gu lan and diabetes

[Guest guest]

From pubmed

A novel insulin-releasing substance, phanoside, from the plant

gynostemma pentaphyllum.

 

Norberg A, Hoa NK, Liepinsh E, Phan DV, Thuan ND, Jornvall H,

Sillard R, Ostenson CG.

 

MBB, Karolinska Institutet, Stockholm 171 77.

 

Extracts from Gynostemma pentaphyllum Makino (Cucurbitaceae), an

East-Asian herb, has been reported to have numerous activities, such

as antitumor, cholesterol-lowering, immunopotentiating, antioxidant

and hypoglycemic effects. We have isolated one active compound by

ethanol extraction, distribution in butanol/water, solid phase

extraction/separation, and several rounds of RP-HPLC. We show by NMR

and mass spectrometry that it is a novel saponin, a gypenoside that

we name phanoside, (21-,23-epoxy-,3beta-,20-,21-trihydroxydammar-24-

ene-3-O-{[alpha-D-rhamnopyranosyl(1-2)]-[beta-D-glycopyranosyl(1-3)]-

beta-D-lyxopyranoside} with a molecular mass of 914.5 Da. Phanoside

is a dammarane-type saponin, and 4 stereoisomers differing in

configurations at positions 21 and 23 were identified, each of which

were found to stimulate insulin release from isolated rat pancreatic

islets. We also found that the stereoisomers are interconvertible.

Dose-dependent insulin-releasing activities at 3.3 mM and 16.7 mM

glucose levels were determined for the racemic mixture containing

all 4 stereoisomers. Phanoside at 500 microM stimulates insulin

release in vitro 10-fold at 3.3 mM glucose and potentiates the

release almost 4-fold at 16.7 mM glucose. At these glucose levels, 2

microM glibenclamide stimulates insulin release only 2-fold.

Interestingly, beta-cell sensitivity to phanoside is higher at 16.7

mM than at 3.3 mM glucose, since insulin responses were

significantly increased by phanoside below 125 M only at high

glucose levels. Also, when given orally to rats, phanoside (at 40

and 80 mg/kg) improved glucose tolerance and enhanced plasma insulin

levels at hyperglycemia.

[Guest guest]

Has anyone used Gynostema on diabetic patients?I have ready access to a formula

for this

and a number of diabetic patients but haven't tried it yet without some clinical

evidence.

Doug

 

, " richblit " <richblit@r...> wrote:

> From pubmed

> A novel insulin-releasing substance, phanoside, from the plant

> gynostemma pentaphyllum.

>

> Norberg A, Hoa NK, Liepinsh E, Phan DV, Thuan ND, Jornvall H,

> Sillard R, Ostenson CG.

>

> MBB, Karolinska Institutet, Stockholm 171 77.

>

> Extracts from Gynostemma pentaphyllum Makino (Cucurbitaceae), an

> East-Asian herb, has been reported to have numerous activities, such

> as antitumor, cholesterol-lowering, immunopotentiating, antioxidant

> and hypoglycemic effects. We have isolated one active compound by

> ethanol extraction, distribution in butanol/water, solid phase

> extraction/separation, and several rounds of RP-HPLC. We show by NMR

> and mass spectrometry that it is a novel saponin, a gypenoside that

> we name phanoside, (21-,23-epoxy-,3beta-,20-,21-trihydroxydammar-24-

> ene-3-O-{[alpha-D-rhamnopyranosyl(1-2)]-[beta-D-glycopyranosyl(1-3)]-

> beta-D-lyxopyranoside} with a molecular mass of 914.5 Da. Phanoside

> is a dammarane-type saponin, and 4 stereoisomers differing in

> configurations at positions 21 and 23 were identified, each of which

> were found to stimulate insulin release from isolated rat pancreatic

> islets. We also found that the stereoisomers are interconvertible.

> Dose-dependent insulin-releasing activities at 3.3 mM and 16.7 mM

> glucose levels were determined for the racemic mixture containing

> all 4 stereoisomers. Phanoside at 500 microM stimulates insulin

> release in vitro 10-fold at 3.3 mM glucose and potentiates the

> release almost 4-fold at 16.7 mM glucose. At these glucose levels, 2

> microM glibenclamide stimulates insulin release only 2-fold.

> Interestingly, beta-cell sensitivity to phanoside is higher at 16.7

> mM than at 3.3 mM glucose, since insulin responses were

> significantly increased by phanoside below 125 M only at high

> glucose levels. Also, when given orally to rats, phanoside (at 40

> and 80 mg/kg) improved glucose tolerance and enhanced plasma insulin

> levels at hyperglycemia.