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Black Cumin ** Experiment I

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Journal of Ethnopharmacology

Volume 81, Issue 2, pp. 161 - 164, July, 2002

Nigella sativa oil, nigellone and derived thymoquinone inhibit synthesis

of 5-lipoxygenase products in polymorphonuclear leukocytes from rats

Authors

 

M. El-Dakhakhny, N.J. Madi, N. Lembert, H.P.T. Ammon

 

Abstract

 

In the present study, Nigella sativa oil (NSO), nigellone

(polythymoquinone) and derived thymoquinone were studied to evaluate

their effect on the formation of 5-lipoxygenase (5-LO) products from

polymorphonuclear leukocytes (PMNL).NSO produced a concentration

dependent inhibition of 5-LO products and 5-hydroxy-eicosa-tetra-enoic

acid (5-HETE) production with half maximal effects (IC50) at 25+/-1

µg/ml, respectively 24+/-1 µg/ml. Nigellone caused a concentration

related inhibition of 5-HETE production (IC50: 11.9+/-0.3 µg/ml).

 

Moreover thymoquinone, the active principle of NSO inhibited the

production of 5-LO products (IC50: 0.26+/-0.02 µg/ml) and 5-HETE

production (IC50: 0.36+/-0.02 µg/ml) in a similar way. The effects are

probably due to an antioxidative action. The data may in part explain

the effect of the oil, its derived thymoquinone and nigellone in

ameliorating inflammatory diseases.

 

Keywords: Nigella sativa, Nigellone, Thymoquinone, 5-LO products

 

PII: s037887410200051x

 

© Copyright 1999-2003, Elsevier Science, All rights reserved.

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