Cover-up over GM DNA in milk

[Guest guest]

MORE INFO: http://www.phpbbforfree.com/forums/infonature-post-654.html#654

 

ISIS Press Release 26/07/05

Cover-up over GM DNA in milk

Syngenta's GM maize linked to dead cows linked to GM DNA in milk and

scientist involved in what appears to be a major cover-up on behalf of big dairy

producer. Dr. Mae-Wan Ho

 

A fully referenced version of this article is posted on ISIS members'

website. Details here

 

Campaign against GM animal feed

Greenpeace Germany began campaigning against GM animal feed in March 2005.

Their main target is Mueller, one of Germany's biggest dairy producers and also

number one in Britain in yogurt sales. Greenpeace exposed Mueller's use of GM

soya to feed their dairy cows, which Mueller does not deny. But the company

tried to stop Greenpeace's campaign, and especially the use of the term

" GE-milk " through the law court. The company claims it is scientifically

demonstrated that no GM DNA could transfer into the milk, and produced a

statement signed by six German scientists with the title, No transfer of

genetically modified components from animal feed to milk. Greenpeace contacted

me for help in producing a counter-statement. The counter-statement, Transfer of

genetically modified DNA from animal feed to milk cannot be ruled out, and is a

cause for concern, was eventually signed by Dr. Mae-Wan Ho, Dr. Arpad Pusztai,

Dr. Susan Bardocz, Prof. Joe Cummins and Prof. Peter Saunders on behalf of the

Independent Science Panel (www.indsp.org).

 

On 5 July 2005, the court of Cologne decided in favour of Greenpeace,

refusing to grant Mueller an injunction. The court stated that as since Mueller

is using genetically modified plants for animal feed, the products are connected

with genetic engineering and therefore the term GE-milk is perfectly justified.

Mueller claimed that GM-DNA fragments are not present in milk, but Greenpeace

countered by saying it was not yet scientifically decided.

" The report of the ISP was vital to support our arguments! " says

Greenpeace activist

Ulricke Brendel. Unfortunately, that's not the end of the matter. Mueller

has filed a new case against Greenpeace, going for the highest court in Germany,

to prevent Greenpeace from using the term, " GE-milk " , and also asking for ?500

000 in damage compensation.

 

" For the next 3 to 5 years, that is as long it might take, we will keep

arguing the case, " Ulricke added.

So what's the current status of the evidence? Is there or is there not GM-

DNA in milk?

 

Unpublished evidence kept under lock and key

There are several published studies on the transfer of genetically

modified (GM) DNA from animal feed to milk, all of them methodologically flawed;

nevertheless they indicate that it is possible for DNA from GM feed to transfer

to milk. And this is confirmed in an unpublished study from the Weihenstephen

Institute of Physiology and the Technical University of Munich.

Astonishingly, the lead author of the unpublished study from Weihenstephen

Institute, which found positive evidence of GM DNA in milk - Prof. Rolf Espanier

- is also the lead author of the statement on behalf of the company Mueller,

claiming there is no transfer of genetically modified components from animal

feed to milk.

Furthermore, that unpublished study was done on milk collected from dairy

cows in a farm in Hesse Germany where, between 2000 and 2001, 12 cows died after

eating Syngenta's GM maize Bt 176 ( " Cow ate GM maize and died " , SiS 21). No

proper autopsies were carried out; while this crucial study dated 20 October

2000 remained under lock and key for more than three years before it was leaked

to Greenpeace [1].

 

A handful of studies

The first study in the laboratories of Einspanier, Jahreis and Falchowsky

[2] detected " faint signals " of the abundant plant chloroplast DNA in milk, but

not the GM DNA. However, the limit of detection, i.e., the sensitivity of the

detection method, was not reported. This would involve spiking the milk with

increasing amounts of DNA from the GM feed until a positive signal is obtained.

 

A second study in another laboratory [3] failed to detect any GM DNA in

milk. But the limit of detection was 30 ng GM soya DNA added to the milk, which

is equivalent to 16 200 copies of the GM soya genome, or the same number of

copies of the GM DNA insert, assuming there is a single insert in the genome.

This is unacceptably high compared to the standard limit of detection of 10

copies or less; and it indicates that the method used was far from sensitive

enough. A follow-up investigation [4] did detect plant chloroplast DNA, but not

the GM DNA in milk. Chloroplast DNA outnumbers GM DNA by up to 50 000 copies to

1. The limit of detection in this study was still unacceptably high; it required

the presence of 2 700 copies of the GM soya genome and 602 copies of the GM

maize genome in 330 microlitres (about three drops) of milk. Another limitation

of these studies was that the feeding trials [2, 3] were of short duration,

lasting only several weeks.

 

The fourth published study [5] established the limit of detection as

between 5 and 10 genomic copies of the GM DNA, but not by adding the GM plant

DNA to milk, which is necessary, as inhibitors of the detection reaction are

often present. Nevertheless these researchers found plant chloroplast DNA in

high proportions, possibly all, of the milk samples from dairy cows: 86%

positives while the rest were 'indeterminate'. They claim to have found " no

statistically significant " presence of GM DNA in milk. No information on the

length of the feeding trial(s) was given.

 

Positive evidence for the transfer of GM DNA into milk was presented in

the unpublished report [6] from Weihenstephen Institute referred to earlier. Two

milk samples were analysed, and in both of them, positive signals for GM DNA

were found.

These studies used a wider range of probes for different plant DNA:

Ubiquitin and zein (about 20 and 40 copies respectively in the maize

genome); EPSPS, single copy gene specific for GM soya; rubisco gene in

chloroplast genome (about 10 000 to 50 000 copies); and Bt (CrylA), single copy

gene specific for GM maize

The first milk sample was probed for ubiquitin, rubisco and Bt; the second

sample was probed for all five gene-sequences. The milk was separated by

centrifugation into the cell fraction at the bottom, fat at the top and solution

in between.

The first sample showed that ubiquitin DNA was present in all the cell and

fat fractions, but not in solution. The chloroplast rubisco DNA could be

detected in all cell and fat fractions. The Bt DNA was detected in all the

fractions that were positive for chloroplast DNA, with a rather similar pattern.

 

The summary stated, " It was not difficult to prove the existence of

general plant DNA (chloroplasts) in this milk. In addition, positive signals for

the presence of Bt-maize fragments were obtained. This data indicates the

presence of small quantities of Bt-maize gene fragments in the tank milk. "

(emphasis added)

 

However, the authors made the unjustified assumptions that the Bt-maize

gene fragments came from other sources than the animals producing the milk and

that they have no biological significance, " The presence of Bt-maize material in

the milk supplied is not necessary due to endogenous factors (i.e., via the

animal itself). Thus, the presence of many different kinds of feed in the tank

milk is likely and almost inevitable in spite of stringent hygienic conditions.

The PCR analysis will also detect dust or aerosols from neighbouring feeding

areas. On the basis of the biological knowledge available to us, the presence of

the very small quantity of Bt-maize DNA identified has only analytical but no

biological relevance whatsoever. "

In the second sample, not only was the Bt gene fragment from GM maize

detected in milk, the EPSPS gene fragment from GM soya - contained in the animal

feed - was also detected. The summary stated, " In this milk, it was possible to

identify sporadic traces of general plant DNA (chloroplasts) as well as zein and

EPSPS gene fragments. As well as this, slightly positive signals indicating the

presence of Bt-maize fragments were also contained. This data indicates minor

contamination with Bt-maize gene fragments in the tank milk. "

 

Again, this " contamination " was deemed to have " no biological relevance

whatsoever. "

 

GM DNA in milk is a cause for concern

The presence of GM DNA in milk is a cause for concern, regardless of

whether it originated in the animal producing the milk, or by contamination from

" dust or aerosols " containing GM feed, which according to the authors of the

unpublished report [6] " is likely and almost inevitable in spite of stringent

hygienic conditions. "

 

GM DNA is unlike natural DNA in many respects [7]. It contains new

combinations of genetic material that have never existed in billions of years of

evolution, including genes sequences that are completely synthesized in the

laboratory, differing significantly from their natural counterparts. GM DNA is

designed with recombination sequences in order to break and insert into genomes;

it also contains other changes to overcome genetic differences between species.

GM DNA inserting into genomes causes mutational and other genome rearrangements

including cancer. In addition, GM DNA contains a high proportion of viral and

bacterial DNA, known to cause a range of immune reactions in human [8].

 

Another source of hazard from GM DNA comes from the gene products encoded,

which have never been part of our food chain. For example, one study found that

two-thirds of all the transgenes have similarities to known allergens [9, 10]

and should be regarded as potential allergens until proven otherwise.

 

 

 

 

 

--

 

This article can be found on the I-SIS website at http://www.i-

sis.org.uk/GMDNAinMilk.php

 

If you like this original article from the Institute of Science in

Society, and would like to continue receiving articles of this calibre, please

consider making a donation or purchase on our website. ISIS is an independent,

not-for-profit organisation dedicated to providing critical public information

on cutting edge science, and to promoting social accountability and ecological

sustainability in science.